cif (for Cut In Frame) is a tool that works with DNA sequences. It is used to digest your sequences with a pool of restriction enzymes and to search which enzymes cut your sequence keeping your reading frame after ligation without any frame shift produced due to the digestion.
This helps users to work with a gene of interest to localize potential region(s) that could be removed from the final protein to check if regions have an impact or not on the final gene product. It can also allow to identify vital region(s) for the gene.

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